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pmxs klf4 addgene  (Addgene inc)


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    Addgene inc pmxs klf4 addgene
    Pmxs Klf4 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmxs klf4 addgene/product/Addgene inc
    Average 93 stars, based on 119 article reviews
    pmxs klf4 addgene - by Bioz Stars, 2026-02
    93/100 stars

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    (A) Correlation between GFP+ colony number and percentage 12 days post transduction. Each dot represents a single well of the reprogramming experiments. (B) Representative FACS plot for <t>SSEA1/Oct4-GFP</t> at 12 days post-viral transduction. (C, D) Relative reprogramming efficiencies are shown, with the fold changes indicated. (C) Fully reprogrammed efficiency, assessed by the percentage of SSEA1+Oct4-GFP+ cells; (D) Intermediately reprogrammed efficiency, assessed by percentage of SSEA1+Oct4-GFP- cells; Student's t-test (two-tailed) is used for statistics. Error bars, standard error. n = experiments with independent MEFs. Ctrl, wild type or heterozygous littermate controls; TKO, triple knockout of piwi.
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    (A) Correlation between GFP+ colony number and percentage 12 days post transduction. Each dot represents a single well of the reprogramming experiments. (B) Representative FACS plot for SSEA1/Oct4-GFP at 12 days post-viral transduction. (C, D) Relative reprogramming efficiencies are shown, with the fold changes indicated. (C) Fully reprogrammed efficiency, assessed by the percentage of SSEA1+Oct4-GFP+ cells; (D) Intermediately reprogrammed efficiency, assessed by percentage of SSEA1+Oct4-GFP- cells; Student's t-test (two-tailed) is used for statistics. Error bars, standard error. n = experiments with independent MEFs. Ctrl, wild type or heterozygous littermate controls; TKO, triple knockout of piwi.

    Journal: PLoS ONE

    Article Title: PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells

    doi: 10.1371/journal.pone.0097821

    Figure Lengend Snippet: (A) Correlation between GFP+ colony number and percentage 12 days post transduction. Each dot represents a single well of the reprogramming experiments. (B) Representative FACS plot for SSEA1/Oct4-GFP at 12 days post-viral transduction. (C, D) Relative reprogramming efficiencies are shown, with the fold changes indicated. (C) Fully reprogrammed efficiency, assessed by the percentage of SSEA1+Oct4-GFP+ cells; (D) Intermediately reprogrammed efficiency, assessed by percentage of SSEA1+Oct4-GFP- cells; Student's t-test (two-tailed) is used for statistics. Error bars, standard error. n = experiments with independent MEFs. Ctrl, wild type or heterozygous littermate controls; TKO, triple knockout of piwi.

    Article Snippet: The following plasmids were obtained from Addgene: pMXs-Oct3/4-IP (15918), pMXs-Sox2-IP (15919), pMXs-Klf4-IP (15920) and pMXs-c-Myc-IP (16921). (Addgene).

    Techniques: Transduction, Two Tailed Test, Triple Knockout

    (A) iPS colonies exhibited typical ES cell morphology and expressed Oct4-GFP homogeneously. (B) Representative figure showing TKO iPS cells expressed comparable level of Oct4-GFP as Ctrl cells. ( n = 20). Relative expression levels of (C) Oct4-GFP and (D) SOX2 proteins, as denoted by quantitative mean fluorescence intensity (MFI) shown by FACS analysis ( n = 3 and 8, respectively). (E) Both TKO and Ctrl cells remained pluripotent and express Oct4-GFP + SSEA1 + (>99%) over 30 passages. (F) A competition strategy was designed to determine if Piwi depletion compromises iPSC self-renewal. GFP+ cells (marked the iPSCs) were mixed in a 1-to-1 ratio with normal mouse ESCs (GFP-) cells, and cultured in the presence of LIF. With each passage the ratio of GFP/total cells was measured by FACS. The proportion of GFP+ cells with TKO were indiscernible from Ctrl cells over five passages (n = 3). Ctrl, wildtype or heterozygous littermate controls; TKO, triple knockout of piwi.

    Journal: PLoS ONE

    Article Title: PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells

    doi: 10.1371/journal.pone.0097821

    Figure Lengend Snippet: (A) iPS colonies exhibited typical ES cell morphology and expressed Oct4-GFP homogeneously. (B) Representative figure showing TKO iPS cells expressed comparable level of Oct4-GFP as Ctrl cells. ( n = 20). Relative expression levels of (C) Oct4-GFP and (D) SOX2 proteins, as denoted by quantitative mean fluorescence intensity (MFI) shown by FACS analysis ( n = 3 and 8, respectively). (E) Both TKO and Ctrl cells remained pluripotent and express Oct4-GFP + SSEA1 + (>99%) over 30 passages. (F) A competition strategy was designed to determine if Piwi depletion compromises iPSC self-renewal. GFP+ cells (marked the iPSCs) were mixed in a 1-to-1 ratio with normal mouse ESCs (GFP-) cells, and cultured in the presence of LIF. With each passage the ratio of GFP/total cells was measured by FACS. The proportion of GFP+ cells with TKO were indiscernible from Ctrl cells over five passages (n = 3). Ctrl, wildtype or heterozygous littermate controls; TKO, triple knockout of piwi.

    Article Snippet: The following plasmids were obtained from Addgene: pMXs-Oct3/4-IP (15918), pMXs-Sox2-IP (15919), pMXs-Klf4-IP (15920) and pMXs-c-Myc-IP (16921). (Addgene).

    Techniques: Expressing, Fluorescence, Cell Culture, Triple Knockout